Assay kits for the estimation of biochemical factor such as aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were purchased from Pars Azma (Iran).Ĭornus mas fruits were obtained from suburbs of kaleibar (East Azarbayjan, Iran) at the end of summer 2012. Carbon tetrachloride (CCl 4) and thiobarbituric acid (TBA) were obtained from Merck Co. This study was designed to investigate the protective effects of Cornus mas fruits extract on serum biomarkers in CCl 4-induced hepatotoxicity in male rats.Įthylene diamine tetra acetic acid (EDTA) and trichloroacetic acid (TCA) were obtained from Sigma-Aldrich Chemical Co. It also contains antioxidant substances including butylated hydroxyanisole and butylated hydroxytoluene and has the potential in the treatment of some cancers ( 24, 25). mas fruits contain iron, calcium, vitamins such as E, B2, B1, C, folic acid, anthocyanins, flavonoids, and plenty of oxalic acid ( 22, 23). This plant has been used in traditional medicine for the treatment of diarrhea, intestinal inflammation, fever, malaria treatment, kidney stones and kidney and bladder infections. mas) is known as the European and Asiatic Cornelian Cherry. Cornus mas fruits have been used for gastrointestinal and excretory disorders in traditional medicine ( 19) also improve liver and kidney functions, and have protective effects such as antiallergic, antidiabetic, antibacterial, antimicrobial, antihistamine and antimalarial properties ( 20, 21).Ĭornus mas ( C. Recently, an increasing trend of herbs usage has been seen, such as cornelian cherry ( Cornus mas), which contains high level of antioxidants and anthocyanins.
#U 26 CORNUS MAS FREE#
Natural antioxidants such as fruits and vegetables, which provide protection against free radicals can decrease the incidence and mortality rates due to cancer and heart diseases, in addition to a number of other health benefits ( 18). Catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GPx) as antioxidative enzymes and glutathione (GSH), vitamins C and E as nonenzymatic antioxidants are biological antioxidants ( 17). Antioxidant and radical scavengers have been studied on the mechanism of CCl 4 toxicity and protect liver cells from CCl 4 induced damage by lipid peroxidation ( 12). When ROS formation is extreme, additional protective mechanisms of dietary antioxidants may be of great importance ( 14, 15).Īntioxidant nutrients and enzymes are the cooperative protective defensive systems against free radical damages ( 16). ROS production and their leading damages would be confined by several endogenous protective mechanisms ( 3). Overproduction of trichloromethyl-free radicals is considered the initial step in a chain of events leading to membrane lipid peroxidation and eventually cell apoptosis or necrosis ( 10- 13). ) from carbon tetrachloride (CCl 4) by hepatic microsomal cytochrome P450, is a well-known model compound for producing chemical hepatic injury ( 5- 9).Biotransformed of trichloromethyl-free radical (CCl 3 During the metabolic processes, free radicals are usually generated especially oxygen-derived radicals with potency of oxidizing and damaging biomolecules ( 4).
Oxygen free radicals and oxygen free-radical generating agents such as hydrogen peroxide (H 2O 2), superoxide anion (O 2 Unsaturated fatty acids of biological cell membranes (a sensitive part of cells against free radicals) may be affected by peroxidation reaction, which leads to a decrease in the fluidity and disruption of membrane function and integrity, and finally serious pathological changes ( 3). The pathogenesis of liver fibrosis is not clear, but reactive oxygen species (ROS) have important roles in liver pathological changes ( 2).
Hepatic injuries lead to attenuation of metabolic functions regulated by liver, and has remained one of the serious health problems ( 1) threatening the human society. Our results validated the traditional use of Cornus mas in the treatment of liver disorders.Ĭarbon Tetrachloride Cornus mas Hepatotoxicity Lipid Peroxidation Serum Biomarkers 1. Conclusions: Cornus mas fruit extract effect may be due to including some antioxidant components, which caused membrane stabilizing and normalization of fluctuated biochemical profiles induced by CCl 4 exposure.
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